DNA fingerprinting and cloning sheet7-mark answer frameWhat is cloning? Explain how Dolly was cloned.
How to lay out the answer. Follow the frame, then write it in your own words.
- 1 · Define. Cloning is making a genetically identical copy of a cell, gene or organism. The copy is a clone.
- 2 · Who and when. Ian Wilmut and Keith Campbell, Roslin Institute, Scotland. Dolly was born in 1996.
- 3 · Write the steps.
- Udder (body) cell from a Finn Dorset sheep
- Egg from a Scottish Blackface ewe, with its nucleus removed (enucleated)
- Fused by a mild electric pulse
- Embryo placed in a surrogate ewe
- Dolly born, identical to the Finn Dorset
- 4 · Draw the flow chart. Donor sheep → udder cell; egg donor → empty egg; fusion; embryo; surrogate; Dolly. Label each box.
- 5 · Merits. Copies of high-yielding animals; saving endangered species; stem cells for treatment.
- 6 · Demerits. Low success rate; clones may fall ill early; ethical concerns.
- 7 · Link to daily life. Banana and sugarcane grown from suckers and cuttings are natural clones.
- 8 · Close in one line. Dolly proved that an adult body cell can produce a whole new animal.
Scoring tip: Name both breeds of sheep correctly and say whose DNA Dolly carried.