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Unit XII · Plant anatomy and plant physiology
6 min readConcept sheetPart II (2 marks) and Part III (4 marks); the diagram can come in Part IV

Internal structure of a dicot leaf

A dicot leaf is dorsiventral: upper and lower epidermis, mesophyll of palisade and spongy parenchyma, and veins with xylem facing up and phloem facing down.

ExampleMango or sunflower leaf
MesophyllPalisade (upper, photosynthesis) + spongy (lower, gas exchange)
StomataMostly on the lower epidermis
Vascular bundleConjoint, collateral, closed; xylem towards the upper side

Tissues of a dicot leaf, from top to bottom

TissueStructureJob
Upper epidermisSingle layer of tightly packed cells with a thick cuticle; few or no stomataProtects; reduces water loss
Palisade parenchymaLong, column-like cells packed closely below the upper epidermis; many chloroplastsMain site of photosynthesis
Spongy parenchymaLoose, irregular cells with large air spaces; fewer chloroplastsExchange of gases; some photosynthesis
Vascular bundles (veins)Conjoint, collateral, closed; xylem towards the upper epidermis, phloem towards the lower; ringed by a bundle sheathXylem brings water; phloem carries food away
Lower epidermisSingle layer with a thin cuticle and many stomata, each with two bean-shaped guard cellsGas exchange and transpiration

Palisade vs spongy parenchyma

Palisade parenchymaElongated cells, closely packed with very few air spaces, many chloroplasts. Lies just below the upper epidermis. Mainly for photosynthesis.
Spongy parenchymaIrregular cells, loosely packed with large air spaces, fewer chloroplasts. Lies above the lower epidermis. Mainly for gas exchange.

Palisade and spongy parenchyma together make up the mesophyll. Because the two sides of the leaf differ, the leaf is called dorsiventral.

How the leaf is built for photosynthesis

  • A thin, flat blade gives a large surface and a short path for light and CO₂.
  • Palisade cells, full of chloroplasts, sit near the top where light is strongest.
  • Air spaces in the spongy tissue let CO₂ reach every cell.
  • Stomata let CO₂ in and O₂ out.
  • Veins bring water in and carry sugar away.

How to draw the T.S. of a dicot leaf

  1. 1
    Draw two long parallel lines a little apart for the leaf blade. Mark a thick line on top: cuticle.
  2. 2
    Under the cuticle, draw one row of box-like cells: upper epidermis.
  3. 3
    Below it, draw one or two rows of tall, closely packed cells with dots (chloroplasts): palisade parenchyma.
  4. 4
    Below that, draw loose, irregular cells with gaps: spongy parenchyma and air space.
  5. 5
    In the middle, draw a vein: a circle with xylem on the upper side and phloem on the lower side, ringed by bundle sheath cells.
  6. 6
    Draw the bottom row of cells: lower epidermis. Break it in two places with a pair of guard cells, and label stoma, guard cell and the sub-stomatal cavity above it.
In real life

Hold a mango or hibiscus leaf: the upper side is darker and shinier (thick cuticle, palisade cells), and the lower side is paler (spongy cells, many stomata).

Exam angle

1 marks: “In a dicot leaf, where are most stomata found?” In the lower epidermis.

2 marks: “Why is a dicot leaf called dorsiventral?” Its mesophyll is divided into palisade parenchyma (upper side) and spongy parenchyma (lower side), so the two surfaces differ.

4 marks: “Describe the internal structure of a dicot leaf.” Upper epidermis with cuticle; palisade parenchyma; spongy parenchyma with air spaces; vascular bundles (xylem up, phloem down, bundle sheath); lower epidermis with stomata; add a labelled diagram.

Remember it

Leaf sandwich, top to bottom: 'Eat Paneer, Skip Eggs': Epidermis (upper), Palisade, Spongy, Epidermis (lower, with stomata).